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Test Catalog

Test ID: MELAN    
Melan A (MART-1) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aids in the identification of melanoma

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Melanoma antigen recognized by T cells or Melan-A (MART-1), is a protein with unknown function that is associated with endoplasmic reticulum and melanosomes. Melan A is a sensitive and specific marker for the diagnosis of melanoma. Melan A is also found in other tumors of melanocytic origin such as clear cell sarcoma, melanotic neurofibroma, melanotic schwannoma, as well as in perivascular epithelioid cell tumor. The monoclonal antibody A103 for Melan A cross-reacts with steroid hormone producing cells and tumors. Consequently, adrenocortical adenomas/carcinomas and sex cord-stromal tumors of the ovary and testis may exhibit staining.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Blessing K, Sanders DSA, Grant JJH:. Comparison of immunohistochemical staining of the novel antibody melan-A with S-100 protein and HMB-45 in malignant melanoma and melanoma variants. Histopathol 1998; 32:139-1462

2. Clarkson KS, Sturdgess IC, Molyneux AJ: The usefulness of tyrosinase in the immunohistochemical assessment of melanocytic lesions: a comparison of the novel T311 antibody (anti-tyrosinase) with S-100, HMB45 and A103 (anti-melan-A). J Clin Pathol 2001;54:196-2003

3. Kaufmann O, Koch S, Gurghardt J, et al: Tyrosinase, melan-A, and KBA62 as markers for the immunohistochemical identification of metastatic amelanotic melanomas on paraffin sections. Mod Pathol 1998;11(8):740-7464

4. Torres-Mora J, Dry S, Li X, et al: Malignant Melanotic Schwannian Tumor: a clinicopathologic, immunohistochemical, and gene expression profiling study of 40 cases, with a proposal for the reclassification of "melanotic schwannoma". Am J Surg Pathol 2014;38(1):94-1055

5. Xu X, Chu AY, Pasha T, et al: Immunoprofile of MITF, tyrosinase, melan-A, and MAGE-1 in HMB-45 negative melanomas. Am J Surg Pathol 2002; 26(1):82-87