Test Catalog

Test ID: PIN2    
PIN2 (p63/p504S) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aids in the identification of high-grade prostatic intraepithelial neoplasia and prostate cancer

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Prostatic intraepithelial neoplasia (PIN) cocktail-2 is a cocktail including 2 antibodies directed against p63 (nuclear) and P504S (cytoplasm) used in the diagnosis of high-grade PIN and prostate cancer. p63 stains the nuclei of normal myoepithelial cells that surround the prostatic epithelial cells. This myoepithelial layer is lost in carcinoma. P504S is abnormally expressed in high-grade PIN and prostate cancer epithelial cells, but is normally expressed in epithelial cells of the renal tubules, gall bladder and bronchi, and in hepatocytes.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Hameed O, Sublett J, Humphrey PA: Immunohistochemical stains for p63 and alpha-methylacyl-CoA racemase, versus a cocktail comprising both, in the diagnosis of prostatic carcinoma: a comparison of the immunohistochemical staining of 430 foci in radical prostatectomy and needle biopsy tissues. Am J Surg Pathol 2005;29(5):579-587

2. Molinie V, Fromont G, Sibony M, et al: Diagnostic utility of a p63/alpha-methyl-CoA-racemase (p504s) cocktail in atypical foci in the prostate. Mod Pathol 2004;17(10):1180-1190

3. Tacha DE, Miller RT: Use of p63/P504S monoclonal antibody cocktail in immunohistochemical staining of prostate tissue. Appl Immunohistochem Mol Morphol 2004 Mar;12(1):75-78