Test Catalog

Test Id : CULFB

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Fibroblast Culture for Biochemical or Molecular Testing, Chorionic Villi/Products of Conception/Tissue

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Producing cell cultures that can be used for enzymatic or molecular genetic analysis

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.

 

To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.

Method Name
A short description of the method used to perform the test

Cell Culture

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Fibroblast Culture for Genetic Test

Aliases
Lists additional common names for a test, as an aid in searching

Cell culture, fibroblasts

Culture for Genetic Testing, Chorionic Villus/CVS

Culture for Genetic Testing, Fibroblasts

Culture for Genetic Testing, Products of Conception/Stillbirth/Autopsy

Culture for Genetic Testing, Tissue

Fibroblast culture for genetic testing

Genetic Testing Culture, Chorionic Villus/CVS

Genetic Testing Culture, Fibroblasts

Genetic Testing Culture, Tissue

Genetic Testing, Culture, Products of Conception/Stillbirth/Autopsy

Saving Cells for Possible Genetic Testing

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.

 

To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.

Specimen Type
Describes the specimen type validated for testing

Tissue

Ordering Guidance

This test is most useful for chorionic villus sampling, products of conception, and skin biopsy specimens. The specimen is cryopreserved for a minimum of 6 months.

 

If ordering this test to obtain DNA for testing at a different laboratory location, see Necessary Information.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells.

Note: For testing performed on fibroblasts at locations other than Mayo Clinic Laboratories (MCL), a MCL Test ID and test requisition for the requested laboratory are required. Prior to specimen collection, call 800-533-1710 to confirm availability of send-out testing options. Courtesy DNA extraction on fibroblast cultures will not be performed for tests not in the MCL referral catalog.

3. Final Disposition of Fetal/Stillborn Remains is required for products of conception or stillbirth specimens.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
CG770 Reason for Referral
CG899 Specimen

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Specimen Type: Autopsy

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 4-mm diameter

Collection Instructions:

1. Wash biopsy site with an antiseptic soap.

2. Thoroughly rinse area with sterile water.

3. Do not use alcohol or iodine preparations.

4. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

 

Specimen Type: Chorionic villi

Supplies: CVS Media (RPMI) and Small Dish (T095)

Container/Tube: 15 mL tube containing 15 mL of transport media

Specimen Volume: 20-30 mg

Collection Instructions:

1. Collect specimen by the transabdominal or transcervical method.

2. Transfer the chorionic villi specimen to a Petri dish containing transport medium.

3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.

 

Specimen Type: Products of conception or stillbirth

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 1 cm(3) of placenta (including 20 mg of chorionic villi) and a 1 cm(3) biopsy specimen of muscle/fascia from the thigh

Collection Instructions: If a fetus cannot be specifically identified, collect 50 mg villus material or tissue that appears to be of fetal origin.

Additional Information: Do not send entire fetus.

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-25 flask

Specimen Volume: 2 Full T-25 flasks

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

1. Biochemical Genetics Patient Information (T602)

2. Final Disposition of Fetal/Stillborn Remains is required for products of conception or stillbirth specimens.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

See Specimen Required

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Tissue Refrigerated (preferred)
Ambient

Useful For
Suggests clinical disorders or settings where the test may be helpful

Producing cell cultures that can be used for enzymatic or molecular genetic analysis

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing chorionic villi, products of conception, skin fibroblasts, or other biopsy specimens for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will be performed. For multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered. Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.

 

To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

If Mycoplasma is detected, all pending fibroblast clinical testing will be canceled, and samples will be discarded.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cultured cells may be used to perform a wide range of laboratory tests. Prior to testing, the tissue may need to be cultured to obtain adequate numbers of cells.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Not applicable

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Interfering factors:

-Inadequate amount of specimen may not permit adequate analysis

-Exposure of the specimen to temperature extremes (freezing or greater than 30 degrees C) may kill cells and interfere with attempts to culture cells

-Improper packaging may result in broken, leaky, and contaminated specimens during transport

-Transport time should not exceed 2 days

-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems

 

Lack of viable cells:

-Bacterial contamination

-Failure to transport tissue in an appropriate media

-Excessive transport time

-Exposure of the specimen to temperature extremes (freezing or >30 degrees C)

-Improper packaging may result in broken or leaky specimen containers or contamination of specimens during transport

Clinical Reference
Recommendations for in-depth reading of a clinical nature

Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. John Wiley and Sons Inc; 2017

Method Description
Describes how the test is performed and provides a method-specific reference

Products of Conception/Autopsy/Stillbirth/Skin Biopsy:

The biopsy specimen is cut into small pieces, treated with collagenase, and placed in a tissue culture flask with Chang and MEM alpha-medium, 20 percent fetal bovine serum, and antibiotics to establish a fibroblast culture. The cultures are trypsinized into 1 to 3 T25 tissue culture flasks or 1 to 2 T75 tissue culture flasks.

 

Chorionic Villi Specimen:

The chorionic villi specimen is thoroughly cleaned using sterile forceps to remove the remaining maternal decidua and blood clots. The villi are then treated with trypsin and collagenase. The cells are grown in Chang and MEM-alpha medium for 5 to 10 days.(May KM, Saxe DF, Priest JH. Prenatal chromosome diagnosis. In: Arsham MS, Barch MJ, Lawce HJ, eds. The AGT Cytogenetics Laboratory Manual. 4th ed. John Wiley and Sons Inc; 2017:182-184)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

41 to 42 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Cell cultures: 6 months; Fresh tissue not utilized to establish cultures: 1 month

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

Not Applicable

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

88233

88240

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
CULFB Fibroblast Culture for Genetic Test 96300-9
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
52327 Result Summary 50397-9
52329 Interpretation 69965-2
52328 Result 82939-0
CG770 Reason for Referral 42349-1
CG899 Specimen 31208-2
52331 Source 31208-2
52332 Method 85069-3
54625 Additional Information 48767-8
52333 Released By 18771-6

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | PHP Pdf | CMS Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports