Detecting drug abuse involving amphetamines, barbiturates, benzodiazepines, cocaine, ethanol, marijuana, opiates, and phencyclidine
This test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
AMPHX | Amphetamines Confirmation, CoC, U | Yes | No |
OPATX | Opiate Confirmation, CoC, U | Yes | No |
BARBX | Barbiturates Confirmation, CoC, U | Yes | No |
COKEX | Cocaine and metabolite Conf, CoC, U | Yes | No |
ETOHX | Ethanol, CoC, U | Yes | No |
PCPX | Phencyclidine Confirmation, CoC, U | Yes | No |
THCX | Carboxy-THC Confirmation, CoC, U | Yes | No |
BNZX | Benzodiazepines Conf, CoC, U | Yes | No |
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
COCH | Chain of Custody Processing | No | Yes |
ADLTX | Adulterants Survey, CoC, U | Yes | Yes |
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If amphetamines, barbiturates, benzodiazepines, cocaine and metabolites, opiates, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry confirmation with quantification will be performed at an additional charge.
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
Alcohol Screened by an Enzymatic Assay/All Others Screened by Immunoassay
7-Amino Flunitrazepam (Metabolite of Flunitrazepam) (Rohypnol)
Alcohol
Alprazolam (Xanax)
Amobarbital (Amytal)
Amphetamines
Amytal (Amobarbital)
Angel Dust (Phencyclidine)
Ativan (Lorazepam)
Barbita (Phenobarbital)
Barbiturates
Benzodiazepines
Benzoylecgonine (Cocaine Metabolite)
Butabarbital (Butisol)
Butalbital (Fiorinal)
Butisol (Butabarbital)
Cannabinoids (Tetrahydrocannabinol)
Chlordiazepoxide (Librium)
Clorazepate (Tranxene)
Cocaine
Codeine
Coke (Cocaine)
Crack (Cocaine)
Dalmane (Flurazepam)
Date Rape Drug (Rohypnol [Flunitrazepam])
Desalkyl Flurazepam (Metabolite)
Desoxyn (Methamphetamines)
Diazepam (Valium)
Dilaudid (Hydromorphone)
Drugs of Abuse
Ecstasy
Ethanol
Ethyl Alcohol
ETOH (Ethanol)
Fiorinal (Butalbital)
Flunitrazepam (Rohypnol)
Flurazepam (Dalmane)
Halicon (Triazolam)
Heroin
Hycodan (Hydrocodone)
Hydrocodone (Hycodan, Vicodin)
Hydromorphone (Dilaudid, Vicodin)
Hydroxy-Ethyl Flurazepam (Metabolite of Flurazepam) (Dalmane)
Killer Weed (Phencyclidine)
Librium (Chlordiazepoxide)
Lorazepam (Ativan)
Lortab (Hydromorphone)
Luminal (Phenobarbital)
Marijuana (Tetrahydrocannabinol)
MDA (Methylenedioxyamphetamine) Metabolite for Methylenedioxyethylamphetamine (MDEA) and Methylenedioxymethamphetamine (MDMA)
MDMA (Methylenedioxymethamphetamine)
Mebaral (Mephobarbital)
Mephobarbital (Mebaral)
Methamphetamines (Desoxyn)
Methylenedioxyamphetamine (MDA) Metabolite for Methylenedioxyethylamphetamine (MDEA) and Methylenedioxymethamphetamine (MDMA)
Methylenedioxymethamphetamine (MDMA)
Morphine
Nembutal (Pentobarbital)
Nordiazepam (Tranxene)
Opiates
Oxazepam (Serax)
Oxycodone (Oxycontin, Percodan)
Oxycontin (Oxycodone)
PCP (Phencyclidine)
Pentobarbital (Nembutal)
Pentothal (Thiopental)
Percodan (Oxycodone)
Phencyclidine (PCP)
Phenobarbital
Restoril (Temazepam)
Rocket Fuel (Phencyclidine)
Rohypnol (Flunitrazepam)
Secobarbital (Seconal)
Seconal (Secobarbital)
Serax (Oxazepam)
Solfoton (Phenobarbital)
Speed (Amphetamines)
TCP (Phencyclidine)
Temazepam (Restoril)
Tetrahydrocannabinol (THC)
THC (Tetrahydrocannabinol)
Thiopental (Pentothal)
Toxicology Screen, Drugs
Tranxene (Clorazepate)
Triazolam (Halcion)
Tuinal (Amobarbital and Secobarbital)
Valium (Diazepam)
Vicodin (Hydrocodone)
Xanax (Alprazolam)
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If amphetamines, barbiturates, benzodiazepines, cocaine and metabolites, opiates, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry confirmation with quantification will be performed at an additional charge.
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
Urine
Container/Tube: Chain-of-Custody Kit (T282) containing the specimen containers, seals, and documentation required
Specimen Volume: 30 mL
Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information: Submitting less than 30 mL will compromise our ability to perform all necessary testing.
1. Chain of Custody Request is included in the Chain-of-Custody Kit (T282).
2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.
15 mL
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 7 days | |
Frozen | 14 days |
Detecting drug abuse involving amphetamines, barbiturates, benzodiazepines, cocaine, ethanol, marijuana, opiates, and phencyclidine
This test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If amphetamines, barbiturates, benzodiazepines, cocaine and metabolites, opiates, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry confirmation with quantification will be performed at an additional charge.
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
This assay was designed to screen for and confirm by gas chromatography-mass spectrometry (GC-MS), gas chromatography-flame ionization detection (GC-FID), or liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the following drugs:
-Amphetamines
-Barbiturates
-Benzodiazepines
-Cocaine
-Ethanol
-Opiates
-Phencyclidine
-Tetrahydrocannabinol
Chain of custody is a record of the disposition of a specimen to document who collected it, who handled it, and who performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.
Negative
Screening cutoff concentrations
Amphetamines: 500 ng/mL
Barbiturates: 200 ng/mL
Benzodiazepines: 100 ng/mL
Cocaine (benzoylecgonine-cocaine metabolite): 150 ng/mL
Ethanol: 10 mg/dL
Opiates: 300 ng/mL
Phencyclidine: 25 ng/mL
Tetrahydrocannabinol carboxylic acid: 50 ng/mL
This report is intended for use in clinical monitoring or management of patients. It is not intended for use in employment-related testing.
A positive result indicates that the patient has used the drugs detected in the recent past. See individual tests (eg, AMPHX / Amphetamines Confirmation, Chain of Custody, Urine) for more information.
For information about drug testing, including estimated detection times, see Drugs of Abuse Testing.
Not intended for use in employment-related testing.
The test does not screen for drug classes other than those listed above. More comprehensive screening is available using the serum or urine drug screens (DSSX / Drug Screen, Prescription/OTC, Chain of Custody, Serum or PDSUX / Drug Screen, Prescription/OTC, Chain of Custody, Urine).
1. Physicians Desk Reference (PDR). 60th edition. Montvale, NJ, Medical Economics Company, 2006
2. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 11th edition. Edited by LL Bruntman. New York, McGraw-Hill Book Company, 2006
3. Langman LJ, Bechtel L, Holstege CP: Chapter 35. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Edited by CA Burtis, ER Ashwood, DE Bruns. WB Saunders Company, 2011, pp 1109-1188
The amphetamines, barbiturates, benzodiazepines, cocaine, opiates, phencyclidine, and tetrahydrocannabinol metabolite assays are based on the kinetic interaction of microparticles in a solution (KIMS) as measured by changes in light transmission. In the absence of sample drug, soluble drug conjugates bind to antibody-bound microparticles, causing the formation of particle aggregates. As the aggregation reaction proceeds in the absence of sample drug, the absorbance increases. When a urine sample contains the drug in question, this drug competes with the drug derivative conjugate for microparticle-bound antibody. Antibody bound to sample drug is no longer available to promote particle aggregation, and subsequent particle lattice formation is inhibited. The presence of sample drug diminishes the increasing absorbance in proportion to the concentration of drug in the sample. Sample drug content is determined relative to the value obtained for a known cutoff concentration of drug.
ADH
Ethyl alcohol + NAD+ -----------------> acetaldehyde + NADH + H+
The NADH formed during the reaction, measured photometrically as a rate of change in absorbance, is directly proportional to the ethyl alcohol concentration.(Package insert: Roche Amphetamines, Barbiturates, Cannabinoids, Benzodiazepines, Cocaine, Ethanol, Opiates, Phencyclidine, Methadone Metabolite reagents, Roche Diagnostic Corp, Indianapolis, IN)
Monday through Saturday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
80307
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
CDAUX | Confirmed Drug Abuse Panel, CoC, U | 87428-9 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
36253 | Amphetamines | 43983-6 |
36254 | Cocaine | 43984-4 |
36255 | Opiates | 70151-6 |
36256 | Phencyclidine | 14310-7 |
36257 | Tetrahydrocannabinol | 14312-3 |
36261 | Chain of Custody | 77202-0 |
36262 | Alcohol | 42242-8 |
36258 | Barbiturates | 70155-7 |
36259 | Benzodiazepines | 14316-4 |