Thyroid transcription factor 1aids in the classification of carcinomas of unknown origin
Keratin 5 aids in the identification of squamous cell carcinoma
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
IHMTO | IHC Multiplex, Tech Only | No | No |
Immunohistochemistry (IHC)
ISTTFK5IHCTO
TECHONLY
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Supplies: Immunostain Technical Only Envelope (T693)
Specimen Type: Tissue
Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block
1. Information on accessing digital images of immunohistochemical (IHC) stains and the manual requisition form can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/
2. Clients ordering stains using a manual requisition form will not have access to digital images.
3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: https://news.mayocliniclabs.com/ihc-stains/#FAQ
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Tissue/Slides | Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
TECHONLY | Ambient (preferred) | ||
Refrigerated |
Thyroid transcription factor 1aids in the classification of carcinomas of unknown origin
Keratin 5 aids in the identification of squamous cell carcinoma
Thyroid transcription factor 1 (TTF1) is a nuclear protein (detected by the chromogen 3,3'-diaminobenzidine: DAB) expressed in thyroid follicular cells, type II pneumocytes, and a subset of bronchial cells. Keratin 5 is a type II cytokeratin (detected by the chromogen fast red) that dimerizes with the type I cytokeratin 14 forming intermediate filaments in the basal layer of the epidermis. Keratin 5 is useful for differentiating squamous cell carcinoma (KRT5 positive) from pulmonary adenocarcinomas (KRT5 negative). This immunostain is often included in a panel to identify the primary site for carcinomas of unknown origin.
This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
1. Kargi A, Gurel D, Tuna B: The diagnostic value of TTF-1, CK 5/6, and p63 immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol Morphol 2007 Dec;15(4):415-420
2. Whithaus K, Fukuoka J, Prihoda TJ, Jagirdar J: Evaluation of napsin A, cytokeratin 5/6, p63, and thyroid transcription factor 1 in adenocarcinoma versus squamous cell carcinoma of the lung. Arch Pathol Lab Med 2012 Feb;136(2):155-162
3. Sterlacci W, Savic S, Schmid T, et al: Tissue-sparing application of the newly proposed IASLC/ATS/ERS classification of adenocarcinoma of the lung shows practical diagnostic and prognostic impact. Am J Clin Pathol 2012;137:946-956
4. Tran L, Mattsson JS, Nodin B, et al: Various Antibody Clones of Napsin A, Thyroid Transcription Factor 1, and p40 and Comparisons with Cytokeratin 5 and p63 in Histopathologic Diagnostics of Non-Small Cell Lung Carcinoma. Appl Immunohistochem Mol Morphol 2016;24:648–659
Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
88344-TC
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
TTFK5 | TTF1(SPT24) + KRT5 IHC, Tech Only | Order only;no result |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
70801 | TTF1(SPT24) + KRT5 IHC, Tech Only | Bill only; no result |