TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: PDCRW    
Pompe Disease Cross-Reactive Immunological Material Status, Leukocytes

Method Description Describes how the test is performed and provides a method-specific reference

Western blot analysis is performed using peripheral blood mononuclear cells (PBMC). Lysed cells are quantitated for protein, separated by gel electrophoresis, and transferred to a polyvinylidene difluoride (PVDF) membrane. The PVDF membrane is then incubated with antibodies specific to acid alpha-glucosidase (GAA, the protein of interest) and b-actin (used as an internal quality control protein). A chemiluminescent substrate is added to the membrane and the emitted light signal is captured by digital imaging. If specific bands are present, the patient is determined to be cross-reactive immunologic material (CRIM)-positive while the absence of these bands indicates a CRIM-negative result.(Wang Z, Okamoto P, Keutzer J: A new assay for fast, reliable CRIM status determination in infantile-onset Pompe disease. Mol Genet Metab. 2014;111:92-100)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester