Test Catalog

Test ID: BTKS    
Bruton Tyrosine Kinase (BTK) Genotype, Full Gene Sequence, Blood

Method Description Describes how the test is performed and provides a method-specific reference

Genomic DNA is first extracted from whole blood, followed by Bruton tyrosine kinase gene (BTK) amplification by PCR. The PCR product is purified from unincorporated primers and nucleotides by enzymatic digestion, and sequenced in both directions using sequencing primers and fluorescent dye-terminator chemistry. Sequencing products are separated on an automated sequencer and trace files are analyzed for variations in the exons and intron/exon boundaries of all 19 exons using specialized mutation detection software  and visual inspection.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.


Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

28 to 42 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Whole Blood: 2 weeks Extracted DNA: 2 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test