Detecting drug abuse involving amphetamines, barbiturates, benzodiazepines, cocaine, ethanol, marijuana, opiates, and phencyclidine
This test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
AMPHU | Amphetamines Confirmation, U | Yes | No |
OPATU | Opiate Confirmation, U | Yes | No |
BARBU | Barbiturates Confirmation, U | Yes | No |
COKEU | Cocaine and metabolite Conf, U | Yes | No |
ETOH | Ethanol, U | No | No |
PCPU | Phencyclidine Confirmation, U | Yes | No |
THCU | Carboxy-THC Confirmation, U | Yes | No |
BNZU | Benzodiazepines Confirmation, U | Yes | No |
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If barbiturates, benzodiazepines, cocaine and metabolites, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry (GC-MS) confirmation with quantification will be performed at an additional charge. Amphetamines and opiates that screen positive will be quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS) at an additional charge.
CDAU: Alcohol Screened by an Enzymatic Assay/All Others Screened by Immunoassay
ETOH: Gas Chromatography-Mass Spectrophotometry (GC-MS)
7-Amino Flunitrazepam (Metabolite of Flunitrazepam) (Rohypnol)
Alcohol
Alprazolam (Xanax)
Amobarbital (Amytal)
Amphetamines
Amytal (Amobarbital)
Angel Dust (Phencyclidine)
Ativan (Lorazepam)
Barbita (Phenobarbital)
Barbiturates
Benzodiazepines
Benzoylecgonine (Cocaine Metabolite)
Butabarbital (Butisol)
Butalbital (Fiorinal)
Butisol (Butabarbital)
Cannabinoids (Tetrahydrocannabinol)
Chlordiazepoxide (Librium)
Clorazepate (Tranxene)
Cocaine
Codeine
Coke (Cocaine)
Crack (Cocaine)
Dalmane (Flurazepam)
Date Rape Drug (Rohypnol [Flunitrazepam])
Desalkyl Flurazepam (Metabolite)
Desoxyn (Methamphetamines)
Diazepam (Valium)
Dilaudid (Hydromorphone)
Drugs of Abuse
Ecstasy
Ethanol
Ethyl Alcohol
ETOH (Ethanol)
Fiorinal (Butalbital)
Flunitrazepam (Rohypnol)
Flurazepam (Dalmane)
Halicon (Triazolam)
Heroin
Hycodan (Hydrocodone)
Hydrocodone (Hycodan, Vicodin)
Hydromorphone (Dilaudid, Vicodin)
Hydroxy-Ethyl Flurazepam (Metabolite of Flurazepam) (Dalmane)
Killer Weed (Phencyclidine)
Librium (Chlordiazepoxide)
Lorazepam (Ativan)
Lortab (Hydromorphone)
Luminal (Phenobarbital)
Marijuana (Tetrahydrocannabinol)
MDA (Methylenedioxyamphetamine) Metabolite for Methylenedioxyethylamphetamine (MDEA) and Methylenedioxymethamphetamine (MDMA)
MDMA (Methylenedioxymethamphetamine)
Mebaral (Mephobarbital)
Mephobarbital (Mebaral)
Methamphetamines (Desoxyn)
Methylenedioxyamphetamine (MDA) Metabolite for Methylenedioxyethylamphetamine (MDEA) and Methylenedioxymethamphetamine (MDMA)
Methylenedioxymethamphetamine (MDMA)
Morphine
Nembutal (Pentobarbital)
Nordiazepam (Tranxene)
Opiates
Oxazepam (Serax)
Oxycodone (Oxycontin, Percodan)
Oxycontin (Oxycodone)
PCP (Phencyclidine)
Pentobarbital (Nembutal)
Pentothal (Thiopental)
Percodan (Oxycodone)
Phencyclidine (PCP)
Phenobarbital
Restoril (Temazepam)
Rocket Fuel (Phencyclidine)
Rohypnol (Flunitrazepam)
Secobarbital (Seconal)
Seconal (Secobarbital)
Serax (Oxazepam)
Solfoton (Phenobarbital)
Speed (Amphetamines)
TCP (Phencyclidine)
Temazepam (Restoril)
Tetrahydrocannabinol (THC)
THC (Tetrahydrocannabinol)
Thiopental (Pentothal)
Toxicology Screen, Drugs
Tranxene (Clorazepate)
Triazolam (Halcion)
Tuinal (Amobarbital and Secobarbital)
Valium (Diazepam)
Vicodin (Hydrocodone)
Xanax (Alprazolam)
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If barbiturates, benzodiazepines, cocaine and metabolites, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry (GC-MS) confirmation with quantification will be performed at an additional charge. Amphetamines and opiates that screen positive will be quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS) at an additional charge.
Urine
For situations where chain of custody is required, a Chain of Custody Kit (T282) is available. For chain-of-custody information, see CDAUX / Drug Abuse Panel with Confirmation, Chain of Custody, Urine.
If urine creatinine is required or adulteration of the sample is suspected, the following test should be requested, ADULT / Adulterants Survey, Urine.
Supplies: Urine Container, 60 mL (T313)
Collection Container/Tube: Plastic urine container
Submission Container/Tube: Plastic, 60-mL urine container (T313)
Specimen Volume: 30 mL
Collection Instructions:
1. Collect a random urine specimen.
2. Submit 30 mL in 1 plastic container.
3. No preservative.
Additional Information:
1. No specimen substitutions.
2. Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.
3. Submitting less than 30 mL will compromise our ability to perform all necessary testing.
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.
15 mL
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 7 days | |
Frozen | 14 days |
Detecting drug abuse involving amphetamines, barbiturates, benzodiazepines, cocaine, ethanol, marijuana, opiates, and phencyclidine
This test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Testing begins with screening assays. If alcohol (ethanol) screen is positive, then the gas chromatography-flame ionization detector confirmation with quantification will be performed at an additional charge. If barbiturates, benzodiazepines, cocaine and metabolites, phencyclidine, or tetrahydrocannabinol metabolite screen is positive, then the gas chromatography-mass spectrometry (GC-MS) confirmation with quantification will be performed at an additional charge. Amphetamines and opiates that screen positive will be quantified with liquid chromatography-tandem mass spectrometry (LC-MS/MS) at an additional charge.
This assay was designed to screen by immunoassay and confirm by gas chromatography-mass spectrometry (GC-MS), gas chromatography-flame ionization detection (GC-FID), or liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the following drugs:
-Amphetamines
-Barbiturates
-Benzodiazepines
-Cocaine
-Ethanol
-Opiates
-Phencyclidine
-Tetrahydrocannabinol
This assay represents the coupling of an immunoassay screen with an automatic confirmation of all positive results by the definitive assay available and described in each individual reflex test ID (eg, AMPHU / Amphetamines Confirmation, Urine). All positive screening results are confirmed by GC-MS, GC-FID, or LC-MS/MS and quantitated before a positive result is reported.
Negative
Screening cutoff concentrations
Amphetamines: 500 ng/mL
Barbiturates: 200 ng/mL
Benzodiazepines: 100 ng/mL
Cocaine (benzoylecgonine-cocaine metabolite): 150 ng/mL
Ethanol: 10 mg/dL
Opiates: 300 ng/mL
Phencyclidine: 25 ng/mL
Tetrahydrocannabinol carboxylic acid: 50 ng/mL
This report is intended for use in clinical monitoring or management of patients. It is not intended for use in employment-related testing.
A positive result indicates that the patient has used the drugs detected in the recent past. See individual tests (eg, AMPHU / Amphetamines Confirmation, Urine) for more information.
For information about drug testing, including estimated detection times, see Drugs of Abuse Testing.
Not intended for use in employment-related testing.
The test does not screen for drug classes other than those listed above. More comprehensive screening is available using the serum or urine drug screens (DSS / Drug Screen, Prescription/OTC, Serum or PDSU / Drug Screen, Prescription/OTC, Urine).
1. Physician's Desk Reference (PDR). 60th edition. Montvale, NJ, Medical Economics Company, 2006
2. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 11th edition. Edited by LL Bruntman. New York, McGraw-Hill Book Company, 2006
3. Langman LJ, Bechtel L, Holstege CP: Chapter 35. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Edited by CA Burtis, ER Ashwood, DE Bruns. WB Saunders Company, 2011, pp 1109-1188
The amphetamines, barbiturates, benzodiazepines, cocaine, opiates, phencyclidine, and tetrahydrocannabinol metabolite assays are based on the kinetic interaction of microparticles in a solution (KIMS) as measured by changes in light transmission. In the absence of sample drug, soluble drug conjugates bind to antibody-bound microparticles, causing the formation of particle aggregates. As the aggregation reaction proceeds in the absence of sample drug, the absorbance increases. When a urine sample contains the drug in question, this drug competes with the drug derivative conjugate for microparticle-bound antibody. Antibody bound to sample drug is no longer available to promote particle aggregation, and subsequent particle lattice formation is inhibited. The presence of sample drug diminishes the increasing absorbance in proportion to the concentration of drug in the sample. Sample drug content is determined relative to the value obtained for a known cutoff concentration of drug.
ADH
Ethyl alcohol + NAD+ -----------------> acetaldehyde + NADH + H+
Monday through Saturday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
80307
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
CDAU | Confirmed Drug Abuse Panel, U | 87428-9 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
30909 | Alcohol | 34180-0 |
2573 | Amphetamines | 43983-6 |
2574 | Barbiturates | 70155-7 |
2575 | Benzodiazepines | 16195-0 |
21652 | Cocaine | 19359-9 |
2577 | Opiates | 18390-5 |
2578 | Phencyclidine | 18392-1 |
2664 | Tetrahydrocannabinol | 19415-9 |
20672 | Chain of Custody | 77202-0 |