TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: F5IS    
Coagulation Factor V Inhibitor Screen, Plasma

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Testing begins with coagulation factor V activity assay with dilutions to evaluate assay inhibition; if the factor V activity assay is normal or increased, the inhibitor screen will be cancelled.  If the factor V activity assay is decreased, an inhibitor screen will be performed to look for specific factor V inhibition. If specific inhibition is apparent, the titer of the inhibitor will be determined.

Specimen Type Describes the specimen type validated for testing

Plasma Na Cit

Advisory Information

This test is for factor V inhibitors only. If the presence or type of inhibitor is unknown, order ALUPP / Lupus Anticoagulant Profile, Plasma first, except for screening studies in patients with known hemophilia A or B.

Shipping Instructions

Send all vials in the same shipping container.

Necessary Information

If priority specimen, mark request form, give reason, and request a call-back.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

See Coagulation Guidelines for Specimen Handling and Processing in Special Instructions.

 

Patient Preparation: Fasting preferred

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 3 mL in 3 plastic vials each containing 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot plasma into 3 separate plastic vials (1 mL in each) leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma immediately (no longer than 4 hours after collection) at -20 degrees C, or, ideally < or =-40 degrees C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

2 mL in 2 plastic vials, 1 mL each

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Plasma Na CitFrozen14 days