Test Catalog

Test ID: BUAUC    
Busulfan, Intravenous Dose, Area Under the Curve, Plasma

Specimen Type Describes the specimen type validated for testing

Plasma Na Heparin

Necessary Information

The time the drug administration is started and completed, the patient's dose (mg every 6 hours), body weight (kg), and age (years) must be submitted with the specimens. Without infusion start time, exact time of specimen collection, dose, and body weight, the area under the curve (AUC) and clearance cannot be calculated. Age is required for assessment of the correct dose per body weight.


A completed Busulfan Information: Mail-In Specimen Instructions (T559) is required. See Special Instructions.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Four plasma specimens with different draw times (keep all specimens under 1 order) are required.


Collection Container/Tube: Green top (sodium heparin)

Submission Container/Tube: Plastic vials

Specimen Volume: 1 mL

Collection Instructions: 

1. The first specimen should be collected immediately after completion of the first intravenous infusion of 0.8 mg/kg busulfan.

2. Additional specimens should also be collected at 1 hour, 2 hours, and 4 hours after completion of infusion.

3. Label each specimen with exact time of collection.

4. Busulfan degrades quickly at ambient temperature. Specimens must be kept in wet ice slurry or refrigerated at 4 degrees C. Specimens must be centrifuged within 2 hours after collection. Separate the plasma and transfer to individual 5-mL plastic vials, labeled with exact time of draw. Immediately freeze at -20 degrees C.

Additional Information:

This test should only be ordered when the following criteria are met:

-Busulfan dosing protocol must be intravenous (IV) administration of 0.8 mg/kg doses every 6 hours over 4 days, for a total of 16 doses

-Specimens must be drawn as described below:

 -1 specimen collected immediately after completion of the first 2-hour IV infusion of busulfan

 -1 specimen collected 1 hour after the infusion is completed

 -1 specimen collected 2 hours after the infusion is completed

 -1 specimen collected 4 hours after the infusion is completed and prior to the next infusion of busulfan

Special Instructions Library of PDFs including pertinent information and forms related to the test


1. Busulfan Information: Mail-In Specimen Instructions (T559) in Special Instructions.

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.25 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Plasma Na HeparinFrozen (preferred)28 days
 Refrigerated 72 hours