Test Catalog

Test ID: AFPA    
Alpha-Fetoprotein, Amniotic Fluid

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If alpha-fetoprotein is positive, then acetylcholinesterase will be performed at an additional charge.

Specimen Type Describes the specimen type validated for testing

Amniotic Fld

Necessary Information

The following information is required:

a. Date ultrasound performed

b. Estimated due date by ultrasound

c. Collection date

d. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.

  If not ordering electronically, provide information on Second Trimester Maternal Screening Alpha-Fetoprotein (AFP)/QUAD Screen Patient Information (T595) and send with specimen.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube: Amniotic fluid container

Specimen Volume: 1 mL

Collection Instructions: Do not centrifuge.

Special Instructions Library of PDFs including pertinent information and forms related to the test


Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
Amniotic FldRefrigerated (preferred)7 days
 Ambient 7 days