Test Id : JCV
JC Virus Detection by In Situ Hybridization
Useful For
Suggests clinical disorders or settings where the test may be helpful
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy
Method Name
A short description of the method used to perform the test
In Situ Hybridization (ISH)
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Aliases
Lists additional common names for a test, as an aid in searching
JCV
JC Virus In Situ Hybridization
John Cunningham Virus (JCV)
GPJCVISH
ISJCVIISH
ISNEGJCVISH
ISDNAJCVISH
PML Virus (Progressive Multi-Focal Leukoencephalopathy)
Specimen Type
Describes the specimen type validated for testing
Special
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Necessary Information
A pathology or diagnostic report (final or preliminary) and brief history are required and must accompany specimen for testing to be performed.
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Tissue
Supplies: Pathology Packaging Kit (T554)
Submit:
Formalin-fixed, paraffin-embedded tissue block (preferred)
OR
4 Unstained glass, positively-charged slides with 5 (+ or -1)-micron formalin-fixed, paraffin-embedded tissue
Additional Information: Broken or cracked unstained slides will be rejected.
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Wet/frozen tissue | Reject |
| Cytology smears | Reject |
| Non-formalin fixed tissue | Reject |
| Nonparaffin embedded tissue | Reject |
| Kidney/renal tissue | Reject |
| Noncharged, ProbeOn, and Snowcoat slides | Reject |
| Broken or cracked unstained slides | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Useful For
Suggests clinical disorders or settings where the test may be helpful
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
JC virus causes progressive multifocal leukoencephalopathy (PML), a rare, often fatal demyelinating disorder of the central nervous system that occurs in individuals with immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease and chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and AIDS.
Interpretation
Provides information to assist in interpretation of the test results
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Studies have shown potential cross reactivity between polyoma (BK) and JC virus in situ hybridization testing.(1)
Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.
The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
Best practices for storage of positively charged slides:
-Minimize time slides are stored after being unpackaged
-Limit exposure to high humidity and heat
-Minimize exposure to plastic
Broken or cracked unstained slides are not compatible with the instrumentation used in our performing laboratories and will be rejected. Additional material may be requested.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Fritzsche FR, Pianca S, Gaspert A, et al. Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol. 2011;20(2):105-110
2. Aksamit AJ, Mourrain P, Sever JL, Major EO. Progressive multifocal leukoencephalopathy: investigation of 3 cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol. 1985;18(4):490-496
3. Aksamit AJ. Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc. 1993;68(9):899-910
4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al. JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol. 2004;63(11):1124-1130
5. Vago L, Cinque P, Sala E, et al. JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol. 1996;12(2):139-146
6. Chen H, Chen XZ, Waterboer T, et al. Viral infections and colorectal cancer: a systematic review of epidemiological studies. Int J Cancer. 2015:137(1)12-24
Method Description
Describes how the test is performed and provides a method-specific reference
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Friday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
88365-Primary
88364-If additional ISH
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| JCV | JC Virus ISH | In Process |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| 71214 | Interpretation | 50595-8 |
| 71215 | Participated in the Interpretation | No LOINC Needed |
| 71216 | Report electronically signed by | 19139-5 |
| 71218 | Material Received | 81178-6 |
| 71596 | Disclaimer | 62364-5 |
| 72115 | Case Number | 80398-1 |