Investigation of immune deficiency due to IgA2 deficiency
Evaluating patients with anaphylactic transfusion reactions
Nephelometry
Serum
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
0.5 mL
Gross hemolysis | OK |
Gross lipemia | Reject |
Gross icterus | OK |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Ambient | 7 days | ||
Frozen | 7 days |
Investigation of immune deficiency due to IgA2 deficiency
Evaluating patients with anaphylactic transfusion reactions
IgA, the predominant immunoglobulin secreted at mucosal
Paradoxically, bacterial infections may also cause IgA deficiency.
IgA deficiency is 1 cause of anaphylactic transfusion reactions.
IgA
0-<5 months: 7-37 mg/dL
5-<9 months: 16-50 mg/dL
9-<15 months: 27-66 mg/dL
15-<24 months: 36-79 mg/dL
2-<4 years: 27-246 mg/dL
4-<7 years: 29-256 mg/dL
7-<10 years: 34-274 mg/dL
10-<13 years: 42-295 mg/dL
13-<16 years: 52-319 mg/dL
16-<18 years: 60-337 mg/dL
> or =18 years: 61-356 mg/dL
IgA1
0-<5 months: 10-34 mg/dL
5-<9 months: 14-41 mg/dL
9-<15 months: 20-50 mg/dL
15-<24 months: 24-58 mg/dL
2-<4 years: 16-162 mg/dL
4-<7 years: 17-187 mg/dL
7-<10 years: 21-221 mg/dL
10-<13 years: 27-250 mg/dL
13-<16 years: 36-275 mg/dL
16-<18 years: 44-289 mg/dL
> or =18 years: 50-314 mg/dL
IgA2
0-<5 months: 0.4-5.5 mg/dL
5-<9 months: 1.5-6.2 mg/dL
9-<15 months: 2.8-7.0 mg/dL
15-<24 months: 3.9-7.7 mg/dL
2-<4 years: 1.3-31.1 mg/dL
4-<7 years: 1.1-39.1 mg/dL
7-<10 years: 1.4-48.0 mg/dL
10-<13 years: 2.6-53.4 mg/dL
13-<16 years: 4.7-55.1 mg/dL
16-<18 years: 6.6-54.3 mg/dL
> or =18 years: 9.7-156.0 mg/dL
Low concentrations of IgA2 with normal IgA1 levels suggest an
Elevated concentrations of IgA2 with normal or low amounts of
Increased total IgA levels also may be seen in benign disorders
Quantitation of specific proteins by nephelometric means may not be possible in lipemic sera due to the extreme light scattering properties of the specimen. Turbidity and particles in the specimen may result in extraneous light scattering signals, resulting in variable specimen analysis.
1. Schauer U, Stemberg F, Rieger CHL, et al: Establishment of
2. Saulsbury FT: Hyperimmunoglobulinemia D and periodic
3. Popovsky MA: Transfusion Reactions. American
In this Siemens Nephelometer II method, the light scattered onto the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume.
A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with an LED, which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement.
The result is calculated by subtracting value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Unpublished Mayo method; Instruction manual: Siemens Nephelometer II, Version 3, Siemens, Inc., Newark, DE, 2008)
Monday, Wednesday, Friday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
82784
82787 x 2
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
IGAS | IgA Subclasses, S | 87552-6 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
IGA_ | IgA | 2458-8 |
IGA1_ | IgA1 | 6886-6 |
IGA2_ | IgA2 | 6939-3 |