Test Catalog

Test Id : EHOLG

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Entamoeba histolytica Antibody, Serum

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

As an adjunct in the diagnosis of extraintestinal, invasive amebiasis

Method Name
A short description of the method used to perform the test

Enzyme-Linked Immunosorbent Assay (ELISA)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

E. histolytica Ab, IgG, S

Aliases
Lists additional common names for a test, as an aid in searching

Amebiasis

Amebic Antibody

Amebic Serology

Amoeba Histolytica

Amoeba, Serum

Amoebiasis Antibody

E Histolytica Antibody, Serum

Entamoeba Histolytica Antibodies

Entamoeba Histolytica Serology

Parasite Serologies-done by clinic

EHOLG

Specimen Type
Describes the specimen type validated for testing

Serum

Ordering Guidance

Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OPE / Ova and Parasite, Travel History or Immunocompromised, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.

Forms

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.4 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Heat-inactivated specimen Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
Frozen 30 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

As an adjunct in the diagnosis of extraintestinal, invasive amebiasis

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Amebiasis is an infection caused by the protozoan parasite, Entamoeba histolytica. The infection is acquired by ingestion of cysts in fecally contaminated food or water; excystation and infection occur in the large intestine. After excystation, trophozoites attach to the intestinal wall and excrete extracellular enzymes that enable invasion of the mucosa and spread to other organs, especially the liver and lung where abscesses may develop.

 

Amebiasis (or amebic dysentery) can cause bloody diarrhea accompanied by fever and prostration. White and red blood cells are found in the stool. Liver abscess can develop several weeks to months later producing hepatomegaly and fever.

 

Serology may be particularly useful in supporting the diagnosis of invasive disease with E histolytica, which is most commonly associated with amebic liver abscess. Serology should not be used to identify or diagnose amebic dysentery due to poor sensitivity in acute, noninvasive disease.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

Reference values apply to all ages.

Interpretation
Provides information to assist in interpretation of the test results

Negative: No antibodies to Entamoeba histolytica detected. This assay is intended for assessment of invasive amebiasis. Repeat testing in 2 to 3 weeks if clinically indicated.

 

Equivocal: Recommend follow-up testing in 10 to 14 days if clinically indicated.

 

Positive: Results are suggestive of current or past infection with Entamoeba histolytica. Direct detection of E histolytica in stool or other specimen sources is recommended to diagnose acute amebiasis.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Previous episodes of intestinal amebiasis may produce a positive serology.

 

Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Bruckner DA. Amebiasis. Clin Microbiol Rev. 1992;5(4):356-369. doi: 10.1128/CMR.5.4.356

2. Petri WA, Haque R, Moonah SN: Entamoeba species, including amebic colitis and liver abscess. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:3273-3286

Method Description
Describes how the test is performed and provides a method-specific reference

Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, a horseradish peroxidase-labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an enzyme-linked immunosorbent assay microwell plate reader.(Package insert: Entamoeba histolytica ELISA IgG Test kit. Gold Standard Diagnostics; GSD-ENTG-120705.F; 01/12/2021)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Tuesday, Thursday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 5 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

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  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86753

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
EHOLG E. histolytica Ab, IgG, S 22285-1
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
EHOLG E. histolytica Ab, IgG, S 22285-1

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | PHP Pdf | CMS Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports