Test Catalog

Test ID: PTP    
Porphyrins, Total, Plasma

Method Description Describes how the test is performed and provides a method-specific reference

The plasma porphyrins profile is performed as a 2-step analysis. First, the total plasma porphyrins concentration is determined by extracting the porphyrins from plasma with a mixture of ethyl acetate and acetic acid. The porphyrins are then back extracted into dilute hydrochloric acid. Total porphyrins are quantified on this extract via scanning spectrofluorometry. If the total plasma porphyrin concentration is elevated, the extract is submitted for HPLC analysis, whereby the individual porphyrin analytes are separated by differential partitioning between a stationary aliphatic surface and a moving aqueous buffer solution. As the porphyrins emerge separately from the column, the quantity of each is monitored with a fluorescence detector.(Ford RE, Ou C, Ellefson RD: Liquid chromatographic analysis for urinary porphyrins. Clin Chem 1981;27:397-401)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday; 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days (not reported Saturday or Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

5 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test