Assessing erythropoietic bone
Flow Cytometry
Whole Blood EDTA
Specimen must arrive within 48 hours of draw.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.
1.5 mL
Gross hemolysis | Reject |
Other | Clotted |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Refrigerated (preferred) | 48 hours | |
Ambient | 24 hours |
Assessing erythropoietic bone
Reticulocytes are immature erythrocytes (RBC) that have been
% RETICULOCYTES
1-3 days: 3.47-5.40%
4 days-4 weeks: 1.06-2.37%
5 weeks-7 weeks: 2.12-3.47%
8 weeks-5 months: 1.55-2.70%
6 months-23 months: 0.99-1.82%
24 months-5 years: 0.82-1.45%
6-11 years: 0.98-1.94%
12-17 years: 0.90-1.49%
Adults: 0.60-2.71%
ABSOLUTE RETICULOCYTES
1-3 days: 147.5-216.4 x 10(9)/L
4 days-4 weeks: 51.3-110.4 x 10(9)/L
5 weeks-7 weeks: 51.8-77.9 x 10(9)/L
8 weeks-5 months: 48.2-88.2 x 10(9)/L
6 months-23 months: 43.5-111.1 x 10(9)/L
24 months-5 years: 36.4-68.0 x 10(9)/L
6-11 years: 42.4-70.2 x 10(9)/L
12-17 years: 41.6-65.1 x 10(9)/L
Adults: 30.4-110.9 x 10(9)/L
The reticulocyte count is a measure of the number of RBCs delivered by the bone marrow. It is elevated with active erythropoiesis such as regeneration, and is decreased in hypoplastic or deficiency conditions such as vitamin B12 deficiency.
Reticulocyte counts must be carefully correlated with other clinical and laboratory findings.
Clotted specimens yield unreliable results and are unacceptable for analysis.
1. Adeli K, Raizman J, Chen Y, et al: Complex biological profile of hematologic markers across pediatric, adult, and geriatric ages: establishment of robust pediatric and adult reference intervals on the basis of the Canadian Health Measures Survey. Clin Chem 2015 Aug;61(8):1075-1086
2. CLSI. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline, Third Edition. CLSI document EP28-A3c. Wayne, PA, Clinical and Laboratory Standards Institute, 2008
3. Soldin J, Brugnara C, Wong EC: Pediatric Reference Intervals. Fifth edition. AACC Press. Washington DC 2005. ISBN 1-594250-32-4
4. Clinical Hematology: Principles, procedures, correlations. Second edition. Edited by CA Lotspeich-Steininger, EA Stiene-Martin, JA Koepke. Philadelphia, Lippincott-Raven, 1998, pp 114-117
The Sysmex XN 9000 reticulocyte analyzer is a flow cytometer that uses an argon laser as the light source. Whole blood specimens are stained with polymethine fluorescent dye and passed through the laser beam in a sheath flow cell. Each blood cell causes a forward light scatter that depends on cell size. This measurement permits a separation of the various blood cell types so that only erythrocytes are included in the cell count. Erythrocytes containing the protein reticulin (ie, reticulocytes) are stained by the dye in the laser beam. The right-angle fluorescence is measured to count these stained cells. The instrument counts 30,000 erythrocytes in each specimen. Because a known aliquot is counted, an actual RBC per unit volume is performed and an absolute reticulocyte count is obtained.(Instruction manual: Automated Hematology Analyzer XN series [XN-9000], North American edition, Code No. CJ410539. November 2015)
Monday through Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
85045
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
RTIC | Reticulocytes, B | 50262-5 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
PRTIC | Reticulocytes, B | 17849-1 |
ARTIC | Absolute Reticulocyte | 60474-4 |